{"id":1237,"date":"2023-09-26T15:01:36","date_gmt":"2023-09-26T18:01:36","guid":{"rendered":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/2023\/09\/26\/023-co-1-microcine-staurosporine-treatment-in-hipsc-derived-motor-neurons-produce-gaps-in-the-spectrin-lattice-of-axons\/"},"modified":"2023-09-26T17:51:51","modified_gmt":"2023-09-26T20:51:51","slug":"023-co-1-microcine-staurosporine-treatment-in-hipsc-derived-motor-neurons-produce-gaps-in-the-spectrin-lattice-of-axons","status":"publish","type":"post","link":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/2023\/09\/26\/023-co-1-microcine-staurosporine-treatment-in-hipsc-derived-motor-neurons-produce-gaps-in-the-spectrin-lattice-of-axons\/","title":{"rendered":"023 + CO-1-Microcine | Staurosporine treatment in hiPSC-derived motor neurons produce gaps in the spectrin lattice of axons"},"content":{"rendered":"<p>Cellular and Molecular Neurobiology<\/p>\n<p><strong>Author:<\/strong> Nahir Guadalupe Gazal | <strong>Email:<\/strong> nggazal@immf.uncor.edu<\/p>\n<hr \/>\n<p>Nahir Gazal <sup>1\u00b0<\/sup>, Mar\u00eda Castellanos-Montiel <sup>2\u00b0<\/sup>, Guillermina Bruno <sup>1\u00b0<\/sup>, Lale Gursu <sup>2\u00b0<\/sup>, Ghazal Haghi <sup>2\u00b0<\/sup>, Gilles Maussion <sup>2\u00b0<\/sup>, Thomas Durcan <sup>2\u00b0<\/sup>, Nicol\u00e1s Unsain <sup>1\u00b0<\/sup><\/p>\n<p>1\u00b0 Instituto de Investigaci\u00f3n M\u00e9dica Mercedes y Mart\u00edn Ferreyra (INIMEC), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba, Argentina<br \/>\n2\u00b0 Early Drug Discovery Unit, Montreal Neurologial Institute-Hospital, Canad\u00e1<\/p>\n<p>The membrane-associated periodic skeleton (MPS) is a protein structure of actin &#8220;rings&#8221; located transversely to the axon and separated every 190 nm by &#8220;spacers&#8221; of ?\/?-spectrin tetramers. In mature neurons, the MPS is organized along almost the entire axonal shaft that correlates with ?II-spectrin\u0092s homogenous distribution. During the maturation of human induced Pluripotent Stem Cells (hiPSCs) in culture, we observed an intriguing interruption in the otherwise uniform distribution of ?II-spectrin along axons, referred to as ?II-spectrin gaps (?IIs-gaps). These appear as stretches devoid of ?II-spectrin. To determine if ?IIs-gaps are associated with axonal constriction or loss, phase contrast and co-immunofluorescence analysis against various cytoskeletal and membrane proteins demonstrated that the lack of ?II-spectrin is specific and that the axon shows no changes in those areas. Consequently, we subjected 2-week-old cultures to acute stress using sub-lethal doses of staurosporine, arsenite and L-glutamate. Remarkably, a significant increase in the occurrence of axons with ?IIs-gaps under staurosporine treatment was observed. STED microscopy of ?II-spectrin showed that the MPS is unaffected outside of the ?IIs-gaps. Staurosporine was also found to induce ?IIs-gaps in dorsal root ganglion neurons, but not in hippocampal neurons, derived from mouse embryos. We believe the study of ?IIs-gaps will provide valuable insights into the formation and dynamics of the MPS in axons.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Author: Gazal Nahir Guadalupe | email: nggazal@immf.uncor.edu | Cellular and Molecular Neurobiology<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17],"tags":[18],"class_list":["post-1237","post","type-post","status-publish","format-standard","hentry","category-session-1","tag-cellular-and-molecular-neurobiology"],"_links":{"self":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1237","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/comments?post=1237"}],"version-history":[{"count":3,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1237\/revisions"}],"predecessor-version":[{"id":1704,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1237\/revisions\/1704"}],"wp:attachment":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/media?parent=1237"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/categories?post=1237"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/tags?post=1237"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}