Disorders of the Nervous System<\/p>\n
Author:<\/strong> Dalila Noelia Jazm\u00edn Mancino | Email:<\/strong> dmancino@immf.uncor.edu<\/p>\n Dalila Noelia Jazm\u00edn Mancino 1\u00b0<\/sup>, Agust\u00edn Anastas\u00eda 1\u00b0<\/sup>2\u00b0<\/sup><\/p>\n 1\u00b0 Instituto de Investigaci\u00f3n M\u00e9dica Mercedes y Mart\u00edn Ferreyra – INIMEC-CONICET-UNC, C\u00f3rdoba, Argentina A polymorphism in Brain Derived Neurotrophic Factor (BDNF) gene at \u224825% frequency leads to the substitution of Valine with Metionine at position 66 (Val66Met) in the sequence of the prodomain of BDNF (pBDNF) and is associated with cognitive deficits. Although little is known about the mechanisms through which this variant affects human behaviour, it has been demonstrated that pBDNF Met induces growth cone retraction and morphological changes in dendrites and dendritic spines, both in vitro and in vivo in neurons of the CNS. We propose that pBDNF Met induces structural alterations in CNS circuits by perturbing intracellular protein trafficking. To test this, pBDNF Val and pBDNF Met expression vectors were cloned into neurons. Initial optimization of plasmid transfections was conducted in both Hek293 cell cultures and 7 DIV neurons. Preliminary analysis has demonstrated that pBDNF induces cell death. Characterization involved quantifications of pyknotic nuclei, along with biochemical and immunofluorescence analysis of active caspase 3 and c-Jun N-terminal kinases (JNK). Ongoing experiments seek optimal timing for studying the impact of pBDNF on intracellular trafficking prior to the cell death. Once determined, a synchronization system of the secretory pathway in cultured neurons will be employed to study protein trafficking within the endomembrane system. These experiments will enhance understanding the mechanisms behind vulnerability to CNS disorders in pBDNF Met carriers.<\/p>\n","protected":false},"excerpt":{"rendered":" Author: Mancino Dalila Noelia Jazm\u00edn | email: dmancino@immf.uncor.edu | Disorders of the Nervous System<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17],"tags":[22],"class_list":["post-1321","post","type-post","status-publish","format-standard","hentry","category-session-1","tag-disorders-of-the-nervous-system"],"_links":{"self":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1321","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/comments?post=1321"}],"version-history":[{"count":4,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1321\/revisions"}],"predecessor-version":[{"id":2250,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1321\/revisions\/2250"}],"wp:attachment":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/media?parent=1321"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/categories?post=1321"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/tags?post=1321"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n
\n2\u00b0 Instituto de Ciencias Biom\u00e9dicas de C\u00f3rdoba (IUCBC), C\u00f3rdoba, Argentina<\/p>\n