Cellular and Molecular Neurobiology<\/p>\n
Author:<\/strong> Paula Gonzalez | Email:<\/strong> pgonzalez@iib.unsam.edu.ar<\/p>\n Paula M. Gonz\u00e1lez 1\u00b0<\/sup>, Rodolfo N. Sanchez Iazurlo 1\u00b0<\/sup>, Paula A. Aguirre 1\u00b0<\/sup>, Barbara Di Marco 3\u00b0<\/sup>, Julieta Alfonso 3\u00b0<\/sup>, Juan Pablo Fededa 1\u00b0<\/sup><\/p>\n 1\u00b0 Instituto de Investigaciones Biotecnol\u00f3gicas, Universidad Nacional de San Mart\u00edn (UNSAM) \u0096 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Emerging evidence suggests that microRNAs, critical post-transcriptional regulators of mRNA translation and stability, plays a crucial role in dendritic and synaptic development. Author: Gonzalez Paula | email: pgonzalez@iib.unsam.edu.ar | Cellular and Molecular Neurobiology<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[34],"tags":[18],"class_list":["post-1890","post","type-post","status-publish","format-standard","hentry","category-session-2","tag-cellular-and-molecular-neurobiology"],"_links":{"self":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1890","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/comments?post=1890"}],"version-history":[{"count":1,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1890\/revisions"}],"predecessor-version":[{"id":2039,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1890\/revisions\/2039"}],"wp:attachment":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/media?parent=1890"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/categories?post=1890"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/tags?post=1890"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
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\n2\u00b0 Escuela de Bio y Nanotecnolog\u00edas (EByN), Universidad Nacional de San Mart\u00edn.
\n3\u00b0 3.Department of Clinical Neurobiology, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, 69120 Heidelberg, Germany<\/p>\n
\nHere, we investigated the role of miR-191, a microRNA implicated in neurodegenerative diseases that is expressed in the murine brain cortex starting from peak cortical neurogenesis.
\nTo elucidate the role of miR-191-5p function, we generated a novel approach using the CRISPR\/Cas9 system delivered via intra-ventricular injections of the pan-neuronal PHP.eB adeno-associated virus (AAV), to deplete miR-191-5p in the early postnatal mice brain by editing the dicer processing bulge site.
\nUsing Golgi-Cox staining coupled with confocal microscopy to assess the morphology of cortical interneurons by Sholl analysis, we found that depletion of miR-191-5p significantly shortened the average length of neuronal processes and reduced the total surface area of the dendritic tree. Additionally, cortical neurons exhibited decreased overall complexity under miR-191-5p depletion, which is consistent with a role for miR-191-5p in dendritic development. Tracing the processes of in vitro miR-191-5p depleted vs control N2A cells showed similar results.
\nOverall, this data suggests that miR-191-5p act as a positive regulator of neuronal branching during brain development. Further experimentation could unravel the regulatory mechanisms governing the observed phenotypes in vivo.<\/p>\n","protected":false},"excerpt":{"rendered":"