{"id":1945,"date":"2023-09-26T21:42:45","date_gmt":"2023-09-27T00:42:45","guid":{"rendered":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/2023\/09\/26\/134-histone-methyltransferases-plays-a-fundamental-role-on-nicotine-preference-in-zebrafish\/"},"modified":"2023-09-26T22:59:50","modified_gmt":"2023-09-27T01:59:50","slug":"134-histone-methyltransferases-plays-a-fundamental-role-on-nicotine-preference-in-zebrafish","status":"publish","type":"post","link":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/2023\/09\/26\/134-histone-methyltransferases-plays-a-fundamental-role-on-nicotine-preference-in-zebrafish\/","title":{"rendered":"134 | Histone Methyltransferases Plays a Fundamental role on Nicotine Preference in Zebrafish"},"content":{"rendered":"<p>Cognition, Behavior, and Memory<\/p>\n<p><strong>Author:<\/strong> Joaquin Ortiz | <strong>Email:<\/strong> joaquinortiz22.jo@gmail.com<\/p>\n<hr \/>\n<p>Joaquin Ortiz <sup>1\u00b0<\/sup>, Leandro Rocco <sup>1\u00b0<\/sup>, Maria Paula Faillace <sup>1\u00b0<\/sup>, Ramon Bernabeu <sup>1\u00b0<\/sup><\/p>\n<p>1\u00b0 IFIBIO-Houssay, UBA-CONICET<\/p>\n<p>Psychostimulants regulate behavioral responses in zebrafish via epigenetic mechanisms. We have previously shown that DNA methylation and histone acetylation inhibition abolish nicotine-induced conditioned place preference (CPP) but little is known about the role of histone methylation in addictive-like behaviors. To assess the influence of histone methylation on nicotine-CPP, zebrafish were treated with a histone 3 (H3) lysine-9 (K9) dimethyltransferase G9a\/GLP inhibitor, BIX-01294 (BIX), which was administered before conditioning sessions. We observed a dual effect of the inhibitor BIX: at high doses inhibited while at low doses potentiated nicotine reward. Transcriptional expression of ?6 and ?7subunits of the nicotinic acetylcholine receptor and of G9a, DNA methyl transferase-3, and HDAC-1 were upregulated in zebrafish with positive scores for nicotine-CPP. BIX treatment per s\u00e9 did not affect transcriptional levels of epigenetic enzymes that regulate trimethylation or demethylation of H3. BIX reduced H3K9me2 protein levels in a dose-dependent manner in key structures of the reward pathway. Our data demonstrate that H3 methylation catalyzed by G9a\/GLP is involved in nicotine-CPP induction. Dimethylation of K9 at H3 is an important epigenetic modification that should be considered as a potential therapeutic target to treat nicotine reward and perhaps other drug addictions.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Author: Ortiz Joaquin | email: joaquinortiz22.jo@gmail.com | Cognition, Behavior, and Memory<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[34],"tags":[20],"class_list":["post-1945","post","type-post","status-publish","format-standard","hentry","category-session-2","tag-cognition-behavior-and-memory"],"_links":{"self":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1945","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/comments?post=1945"}],"version-history":[{"count":1,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1945\/revisions"}],"predecessor-version":[{"id":2099,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1945\/revisions\/2099"}],"wp:attachment":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/media?parent=1945"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/categories?post=1945"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/tags?post=1945"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}