{"id":1971,"date":"2023-09-26T21:42:46","date_gmt":"2023-09-27T00:42:46","guid":{"rendered":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/2023\/09\/26\/186-tgf-gene-therapy-attenuates-behavioral-impairments-and-manifestations-of-dopaminergic-neurodegeneration-induced-by-6-hydroxydopamine-in-rats\/"},"modified":"2023-09-26T23:10:53","modified_gmt":"2023-09-27T02:10:53","slug":"186-tgf-gene-therapy-attenuates-behavioral-impairments-and-manifestations-of-dopaminergic-neurodegeneration-induced-by-6-hydroxydopamine-in-rats","status":"publish","type":"post","link":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/2023\/09\/26\/186-tgf-gene-therapy-attenuates-behavioral-impairments-and-manifestations-of-dopaminergic-neurodegeneration-induced-by-6-hydroxydopamine-in-rats\/","title":{"rendered":"186 | TGF-\u03b2 Gene Therapy Attenuates Behavioral Impairments and Manifestations of Dopaminergic Neurodegeneration induced by 6-hydroxydopamine in rats"},"content":{"rendered":"<p>Disorders of the Nervous System<\/p>\n<p><strong>Author:<\/strong> Mat\u00edas J\u00e1vega Cometto | <strong>Email:<\/strong> matias.javega@unc.edu.ar<\/p>\n<hr \/>\n<p>Mat\u00edas J\u00e1vega Cometto <sup>1\u00b0<\/sup>, Leandro Gabriel Champarini <sup>1\u00b0<\/sup>, Aracely Janneth Naranjo Viteri <sup>1\u00b0<\/sup>, Rosana Crespo <sup>1\u00b0<\/sup>, Claudia Beatriz Here\u00f1\u00fa <sup>1\u00b0<\/sup><\/p>\n<p>1\u00b0 Instituto de Farmacolog\u00eda Experimental de C\u00f3rdoba (IFEC-CONICET), Departamento de Farmacolog\u00eda Otto Orsingher, Facultad de Ciencias Qu\u00edmicas, Universidad Nacional de C\u00f3rdoba, C\u00f3rdoba, Argentina<\/p>\n<p>Parkinson\u2019s disease is a neurodegenerative disorder associated with neuroinflammation and characterized by the progressive loss of nigro-striatal dopaminergic neurons. Our study focuses on the striatum area (CPu), where these neurons establish their synapses, and on non-motor symptoms that usually precede the motor deficits that occur in late stages of the disease. We have shown that 3 weeks after bilateral intrastriatal administration of selective neurotoxin 6-hydroxydopamine (6-OHDA) in Wistar male rats, the animals showed cognitive deficits and anxiety-like behavior that preceded motor disabilities. TGF-\u03b23 is a cytokine that regulates cell proliferation, neurite outgrowth, and anti-inflammatory effects in the central nervous system. We aimed to generate overexpression of TGF-\u03b23 with therapeutic goals in neuronal and glial cells. The introduction of the rat TGF-\u03b23 coding sequence carried in a recombinant adenoviral vector (rAD-TGF\u03b23) into the cerebrospinal fluid 14 days after 6-OHDA administration, reduced cognitive impairment and anxiety-like behavior evaluated through behavioral tests. Through qRT-PCR and Western blot analysis of CPu samples we studied the expression of inflammatory cytokines and the content of presynaptic proteins. We conclude that rAD-TGF\u03b23 could be a possible therapeutic approach to restore and modulate the mentioned changes in the 6-OHDA Parkinson animal model.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Author: J\u00e1vega Cometto Mat\u00edas | email: matias.javega@unc.edu.ar | Disorders of the Nervous System<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[34],"tags":[22],"class_list":["post-1971","post","type-post","status-publish","format-standard","hentry","category-session-2","tag-disorders-of-the-nervous-system"],"_links":{"self":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1971","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/comments?post=1971"}],"version-history":[{"count":1,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1971\/revisions"}],"predecessor-version":[{"id":2131,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1971\/revisions\/2131"}],"wp:attachment":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/media?parent=1971"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/categories?post=1971"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/tags?post=1971"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}