Neurochemistry and Neuropharmacology<\/p>\n
Author:<\/strong> Natalia Colettis | Email:<\/strong> nataliacolettis@gmail.com<\/p>\n Natalia Claudia Colettis 1\u00b0<\/sup>, Maria Victoria Oberholzer 1\u00b0<\/sup>, Martin Habif 1\u00b0<\/sup>, Daniela Alejandra Salas 1\u00b0<\/sup>, William Klein 2\u00b0<\/sup>, Adriano Sebollela 3\u00b0<\/sup>, Sergio Ferreira 4\u00b0<\/sup>, Diana Alicia Jerusalinsky 1\u00b0<\/sup><\/p>\n 1\u00b0 LaNyN, IBCN, School of Medicine, UBA, Argentina. \u03b2-Amyloid oligomers (A\u03b2Os) were reported as early contributing to synaptic impairment and memory deficits in animal models of Alzheimer’s Disease (AD). A single-chain antibody fragment NUsc1, selectively target a subpopulation of A\u03b2Os and rescued short-term memory (STM) in mice models. To neutralize A\u03b2Os and enhance therapeutic efficacy, we developed an Adeno-Associated virus-derived vector for expressing NUsc1 (V) in the brain. Author: Colettis Natalia | email: nataliacolettis@gmail.com | Neurochemistry and Neuropharmacology<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[34],"tags":[25],"class_list":["post-1995","post","type-post","status-publish","format-standard","hentry","category-session-2","tag-neurochemistry-and-neuropharmacology"],"_links":{"self":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1995","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/comments?post=1995"}],"version-history":[{"count":1,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1995\/revisions"}],"predecessor-version":[{"id":2163,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1995\/revisions\/2163"}],"wp:attachment":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/media?parent=1995"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/categories?post=1995"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/tags?post=1995"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
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\n2\u00b0 Department of Neurobiology, Northwestern University, Evanston, IL 60201, USA.
\n3\u00b0 Department of Biochemistry and Immunology, Ribeirao Preto Medical School, University of S\u00e3o Paulo, Ribeirao Preto 14049-900, Brazil.
\n4\u00b0 Medical Biochemistry Institute & Biophysics Carlos Chagas Filho Institute, UFRJ; Brazil.<\/p>\n
\nThe McGill-R-Thy1-hAPP heterozygous transgenic (Tg+\/\u2013) rat model of AD displays progressive amyloid pathology, impairing the formation\/recall of novel object recognition (NOR) long-term memory (LTM).
\nWe evaluated if early V treatment could rescue NOR LTM in (Tg+\/\u2013) rats. Both Tg and wild-type (wt) male rats 10-12 weeks old, were i.c.v. infused with V or saline (control).
\nAfter two months, exploration to an open field (OF), new object discrimination and recognition (NOR) and LTM were assessed. Both V-treated and control, wt and Tg+\/\u2013 rats showed similar exploratory behavior and habituation to the OF. Tg+\/\u2013 rats failed to express LTM for NOR, whereas prior V treatment restored this capacity
\nCurrent AD therapies underutilize genetic tools. Unlike the already approved ADUCANUMAB and LECANEMAB, expensive antibodies targeting higher-order of A\u03b2 aggregates, V offers a viable, accessible advancement in vector-mediated immunotherapy, enabling sustained scFv NUsc1 neuronal expression to neutralize toxic A\u03b2Os.<\/p>\n","protected":false},"excerpt":{"rendered":"