{"id":1996,"date":"2023-09-26T21:42:48","date_gmt":"2023-09-27T00:42:48","guid":{"rendered":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/2023\/09\/26\/236-evaluating-the-neuroprotective-potential-of-a-new-tetracycline-in-a-novel-cell-model-of-parkinsons-disease\/"},"modified":"2023-09-26T23:23:34","modified_gmt":"2023-09-27T02:23:34","slug":"236-evaluating-the-neuroprotective-potential-of-a-new-tetracycline-in-a-novel-cell-model-of-parkinsons-disease","status":"publish","type":"post","link":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/2023\/09\/26\/236-evaluating-the-neuroprotective-potential-of-a-new-tetracycline-in-a-novel-cell-model-of-parkinsons-disease\/","title":{"rendered":"236 | Evaluating the Neuroprotective Potential of a New Tetracycline in a Novel Cell Model of Parkinson&#8217;s Disease"},"content":{"rendered":"<p>Neurochemistry and Neuropharmacology<\/p>\n<p><strong>Author:<\/strong> Mar\u00eda Laura Guay\u00e1n | <strong>Email:<\/strong> mlauraguayan@gmail.com<\/p>\n<hr \/>\n<p>Mar\u00eda Laura Guay\u00e1n <sup>1\u00b0<\/sup>, Diego Ploper <sup>1\u00b0<\/sup>, Rodrigo Tomas Grau <sup>1\u00b0<\/sup>, Bruno Figad\u00e8re <sup>3\u00b0<\/sup>, Rose Cl\u00e9mence <sup>3\u00b0<\/sup>, Rita Raisman Vozari <sup>2\u00b0<\/sup>, Rosana Cheh\u00edn <sup>1\u00b0<\/sup><\/p>\n<p>1\u00b0 Instituto de Investigaci\u00f3n en Medicina Molecular y Celular Aplicada (IMMCA) (CONICET-UNT-SIPROSA)<br \/>\n2\u00b0 Paris Brain Institute-ICM, Inserm, CNRS, Sorbonne Universit\u00e9 APHP, H\u00f4pital de la Piti\u00e9 la Piti\u00e9-Salp\u00eatri\u00e8re<br \/>\n3\u00b0 BioCIS, Universit\u00e9 Paris-Saclay, CNRS<\/p>\n<p>Parkinson&#8217;s disease (PD) is a chronic neurodegenerative disorder that affects millions worldwide. It involves the progressive loss of dopaminergic neurons in the substantia nigra pars compacta, and is thought to be triggered by the presence of toxic amyloid aggregates of \u03b1-Synuclein (\u03b1S), predominantly phosphorylated at Serine 129 (S129). Currently, no drug is able to stop, nor slow down, neuronal death in PD. Several tetracyclines (TCs) (Minocycline, Doxycycline, Chlortetracycline and Demeclocycline) have shown neuroprotective effects in preclinical models, but their antibacterial activity limits their use. Therefore, modified TCs without antibiotic action were designed and screened for their ability to inhibit \u03b1S aggregation. Here we further evaluate one of the best inhibitors, ChloRed2 (CR2), derived from Chlortetracycline. In SH-SY5Y cells, a PD cellular model, CR2 showed no toxicity and conserved important antioxidant properties present in TCs. In addition, in transgenic SH-SY5Y-\u03b1S-RFP cells, CR2 inhibited the ability of pre-formed \u03b1S fibrils (\u03b1S-PFF) to seed endogenous \u03b1S. Surprisingly, CR2 also inhibited the phosphorylation of \u03b1S at S129 and lysosomal stress induced by \u03b1S-PFF. These results poise CR2 as an attractive drug candidate for further in vivo tests and uncover hidden properties of TCs that could be harnessed for further drug development.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Author: Guay\u00e1n Mar\u00eda Laura | email: mlauraguayan@gmail.com | Neurochemistry and Neuropharmacology<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[34],"tags":[25],"class_list":["post-1996","post","type-post","status-publish","format-standard","hentry","category-session-2","tag-neurochemistry-and-neuropharmacology"],"_links":{"self":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1996","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/comments?post=1996"}],"version-history":[{"count":2,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1996\/revisions"}],"predecessor-version":[{"id":2165,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/1996\/revisions\/2165"}],"wp:attachment":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/media?parent=1996"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/categories?post=1996"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/tags?post=1996"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}