Neuroendocrinology and Neuroimmunology<\/p>\n
Author:<\/strong> Guillermina Canesini | Email:<\/strong> gcanesini@fbcb.unl.edu.ar<\/p>\n Pamela Roc\u00edo Fern\u00e1ndez 1\u00b0<\/sup>, Guillermina Canesini 1\u00b0<\/sup>, Roc\u00edo Schumacher 1\u00b0<\/sup>, Luisa Gaydou 1\u00b0<\/sup>, Mar\u00eda Florencia Rossetti 1\u00b0<\/sup>, Ana Paula Garc\u00eda 1\u00b0<\/sup>, Agustina Sabella 1\u00b0<\/sup>, Jorge Guillermo Ramos 1\u00b0<\/sup>, Cora Stoker 1\u00b0<\/sup><\/p>\n 1\u00b0 Instituto de Salud y Ambiente del Litoral (ISAL), UNL-CONICET, Santa Fe, Argentina. We analysed the impact of neonatal overfeeding and the exposure in adulthood to a cafeteria diet (CAF) on eating behaviour and on the expression of key genes of the reward dopaminergic pathway of the brain. Male Wistar rats were raised in small (4 pups\/dam, SL) or normal litters (10 pups\/dam, NL). From weaning to postnatal day 90 (PND90), they were fed with control diet (CON). From PND90 and for 11 weeks the animals received CON (3 kcal\/g) or cafeteria diet (CAF) (4,85 kcal\/g) (NL-CON, NL-CAF, SL-CON, SL-CAF; 10-14 rats\/group). Body weight and food intake were recorded weekly. Elevated Plus Maze (EPM) and Specific Sensory Satiety test (SSS) were performed. Brains, blood and fat pads were obtained. Ventral Tegmental Area (VTA), was isolated by micropunching technique. For mRNA analysis, qPCR was performed. CAF consumption increased body weight, energy intake and adiposity (p<0,001). CAF groups preferred sweet food (p<0,0001). Eating behaviour in SSS test was altered in SL-CON, NL-CAF y SL-CAF (p<0,05). EPM test results showed anxiety-related behaviour in SL-CAF (p=0,0012). In VTA, SL-CAF enhanced the expression of Tyrosine Hydroxylase (TH) and Dopamine Receptor D2 (DRD2), without altering Dopamine Receptor D1 (DRD1) and Dopamine Active Transporter (DAT) (p<0,05). We are currently studying other nuclei involved in the reward system. This work provides the first evidences that neonatal overfeeding alters eating behaviour in adult life by modifications in the hedonic system.<\/p>\n","protected":false},"excerpt":{"rendered":" Author: Fern\u00e1ndez Pamela Roc\u00edo | email: pame.fernandez@live.com | Neuroendocrinology and Neuroimmunology<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[34],"tags":[26],"class_list":["post-2000","post","type-post","status-publish","format-standard","hentry","category-session-2","tag-neuroendocrinology-and-neuroimmunology"],"_links":{"self":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/2000","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/comments?post=2000"}],"version-history":[{"count":3,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/2000\/revisions"}],"predecessor-version":[{"id":2304,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/posts\/2000\/revisions\/2304"}],"wp:attachment":[{"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/media?parent=2000"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/categories?post=2000"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/csan2023.saneurociencias.org.ar\/index.php\/wp-json\/wp\/v2\/tags?post=2000"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
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\n2\u00b0 C\u00e1tedra de Nutrici\u00f3n en Situaciones Patol\u00f3gicas, FBCB-UNL, Santa Fe, Argentina.
\n3\u00b0 Departamento de Bioqu\u00edmica Cl\u00ednica y Cuantitativa, FBCB-UNL, Santa Fe, Argentina.
\n4\u00b0 C\u00e1tedra de C\u00e1tedra de Morfolog\u00eda Normal, FBCB-UNL, Santa Fe, Argentina<\/p>\n