028 | ilex Paraguariensis (Yerba mate) and Chlorogenic acid increase AMPK phosphorylation and modulate downstream pathway in the neuroblastoma cell line SH-SY5Y

Cellular and Molecular Neurobiology

Author: Hernán Ezequiel Hauché Pedernera | Email: hernanhauche@gmail.com

Hernán Ezequiel Hauché Pedernera , Pedro Ballestero , Tomás Eidelman , Lorena Tschopp , Bordone Melina , Ferrario Juan

1° Laboratorio de Neurobiología de la Enfermedad de Parkinson (IB3, UBA) Ciudad Autónoma de Buenos Aires, Argentina.

Neuroprotection is one of the key challenges in neurodegenerative disorders, therefore, understanding their mechanisms may help develop strategies to delay the process. The identification of neuroprotective compounds enormously helps to reach this goal. Previously we have demonstrated that Yerba Mate (YM) enhances the survival of dopaminergic neurons in primary mesencephalic cultures, similar to green tea and coffee. These beverages have been negatively linked with the development of Parkinson’s disease (PD). They share several active compounds, remarkably polyphenols, such as chlorogenic acid (CGA). To investigate whether YM regulates intracellular mechanisms related to the growth and survival of dopaminergic neurons, we focused on AMPK, a key signaling molecule involved in cell metabolism, strongly linked with neuroprotection, and potentially activated by CGA. Using the simplified model of the SH-SY5Y cell line, we tested the phosphorylation status of AMPK at different concentrations with an extract of YM and CGA. We have found that YM and CGA regulate AMPK phosphorylation. In addition, we will discuss additional preliminary results including the regulation of other regulatory molecules by RT-qPCR and WB. Further work is still necessary to fulfill our hypothesis, but current results settle down the first steps towards understanding how Yerba mate and CGA may modulate neuronal health potentially impacting the progression of neurodegenerative pathologies such as PD.