Title: NeuroTour 2023: A Federal Outlook of Neuroscience in Argentina
Chairs: On behalf of Federalization Commission of the SAN
Gabriela Salvador – INIBIBB-UNS-CONICET, email@example.com
Fernando Gabriel Altamirano – Universidad Nacional de San Luis, firstname.lastname@example.org
Specific Goal of the NeuroTour Symposium
According to the information surveyed by the SAN in 2021, three districts in Argentina concentrate 90% of researchers in the area of Neuroscience: 61% are based in the city of Buenos Aires and 15% and 14% in the provinces of Córdoba and Buenos Aires, respectively. In this context, and within the framework of the recently created Federalization Commission of the SAN, a series of activities were carried out during 2022, including the organization of a pilot experience: the Federal
NeuroTour Symposium at the Annual Meeting.
In this first edition we gathered five researchers from outside the main research nodes representing the provinces of Tucumán, Chaco, Santa Fe, Entre Ríos, and Mendoza. Emphasis was made on inviting non-affiliated speakers and the inclusion of young investigators. We believe that fostering a federal community requires continual work over several years and even decades. For this reason, we present a second edition of the NeuroTour hoping to make this event a tradition within the SAN annual meeting.
As previously mentioned, the main goal of the Federal NeuroTour 2023 is to broaden the neuroscience network along our country. Consequently, the spirit of this symposium was to include several lines of investigation carried out in locations outside the main nodes of SAN. For this reason, in this symposium there is no specific research topic but rather a landscape of neuroscience done out of the most represented areas in SAN. Speakers that will participate in this symposium have their labs in Santa Fe, San Luis, Rio Negro and Tucumán.
Maria Florencia Rossetti Instituto de Salud y Ambiente del Litoral, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral-CONICET- Santa Fe. Website:https://www.scopus.com/authid/detail.uri?authorId=56462267200
Title: “Offspring brain and placental programming in a rodent model of maternal cafeteria diet.”
Abstract: One of the objectives of her research project is to explore the importance of maternal nutritional environments during prenatal and early postnatal life on brain functions and to provide novel mechanisms through which such early experiences may lead to the onset of metabolic syndromes, neurodevelopmental disorders and other brain disorders later in life.
María Florencia Rossetti received her master’s degree in Biotechnology at the University of Litoral (UNL), Santa Fe. She obtained a Ph.D in Biological Sciences at the UNL. Currently, she works at the Instituto de Salud y Ambiente del Litoral, UNL-CONICET and Departamento de Bioquímica Clínica y Cuantitativa, Facultad de Bioquímica y Ciencias Biológicas, UNL in Santa Fe, Argentina.
Fernando Gabriel Altamirano Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis Web https://fergabalt2.wixsite.com/fernando-gabriel-alt
Title: “Chronobiological and epigenetic basis of cognitive functions in an aging model under caloric restriction”
Abstract: Caloric restriction (CR) positively influences aging processes affecting, among others, the cognitive capacities of the hippocampus. Rhythmic variations in memory and associative learning processes throughout the day suggest the participation of the circadian clock in regulating these functions. Despite growing evidence, the molecular basis of CR efficacy has not yet been fully elucidated, nor has the influence of this metabolic context on the temporal and circadian expression of factors related to cognition, antioxidant defense systems and epigenetic regulation remains unexplored.
Fernando Gabriel Altamirano received his master’s degree in Biological Sciences at the Universidad Nacional de San Luis, and he has obtained a Ph.D in Neuroscience (Universidad Nacional de Córdoba). Currently, he is a postdoctoral student at the Instituto de Investigación Médica Martín y Mercedes Ferreyra, working on the role of chromosome imbalance in energetic metabolism and cellular senescence.
María Soledad Espósito Departamento de Física Médica – Centro Atómico Bariloche, CNEA, San Carlos de Bariloche, Rio Negro Website https://www.scopus.com/authid/detail.uri?authorId=56019216600
Title: “Brainstem circuits for motor control in health and disease”
Abstract: Movement is a defining property of the animal kingdom. However, how the nervous system produces diverse and precise actions based on animals’ need continues to be the subject of deep research. This is because execution of diverse movements involves neuronal networks distributed throughout the nervous system. Brainstem motor centers are key components of these networks that have only recently begun to be unraveled establishing that specific brainstem subpopulations embedded into particular upstream and downstream circuits are dedicated to the execution of specific motor programs. The general goal of the lab is to contribute to the understanding of how precise brainstem circuits participate in the generation of particular motor behaviors in health and disease. We are currently focusing on two lines of research. On the one hand, we address the contribution of brainstem circuits to the acquisition of new motor skills. We postulate that motor learning takes place within a multi-level system in which distributed circuit elements, including brainstem circuits, contribute to the formation of a motor memory. Our results support our hypothesis demonstrating that midbrain glutamatergic neurons are necessary for the consolidation of a new motor skill. On the other hand, we are evaluating how particular brainstem circuit dysfunction underlies specific symptoms of Parkinson’s disease. To this end, we have developed a novel model of synucleinopathy which offers new opportunities to study the contribution of individual network elements to disease pathomechanisms.
Maria Soledad Esposito received her master’s degree in Biological Sciences at the University of Buenos Aires (UBA) and she obtained her Ph.D at the Leloir Institute Foundation focused on adult hippocampal neurogenesis. After graduating, she completed a postdoc at the Friedrich Miescher Institute for Biomedical Research in Switzerland. During her postdoc, she worked to characterize the connectivity between the brain and the spinal cord. Currently, she works at the Medical Physics Department at the Centro Atómico Bariloche, Rio Negro, Argentina.
Diego Ploper Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario, Tucumán.Web https://www.scopus.com/authid/detail.uri?authorId=36990454000
Title: “A Transgenic Cellular Model to Probe α-Synuclein Aggregation and Seeding for Parkinson’s Disease”
Abstract: The amyloid aggregation of α-synuclein (α-Syn) within dopaminergic neurons constitutes a pivotal hallmark of Parkinson’s disease (PD). The deleterious accumulation of this protein within dopaminergic neurons is believed to be a key contributor to the disease’s pathogenesis, with intercellular transfer of these aggregates posited as the primary mechanism for disease progression. Here we introduce an enhanced transgenic model designed to investigate α-Syn aggregation and seeding in cultured cells. By utilizing SHSY5Y cells stably overexpressing α-Syn-tagRFP, our study demonstrates that the application of exogenous recombinant human α-Syn preformed fibrils (α-Syn-PFF) elicits an elevation in the count of endogenous α-Syn-tagRFP puncta. Notably, these puncta exhibit positive signals for Thioflavin S (ThS), an amyloid-specific probe, as well as phospho-α-Syn (S129), a hallmark associated with toxic α-Syn species in PD. This observation suggests that exogenous fibrils can serve as seeds to catalyze aggregation of the endogenous protein. Additionally, exogenous α-Syn-PFF induced lysosomal biogenesis, revealing lysosomal stress. Importantly, these effects are confined to α-Syn amyloid fibrils, as exposure to amyloid fibrils originating from other proteins did not influence these parameters. Lastly, as a proof of concept, we illustrate the model’s utility in identifying novel compounds that inhibit α-Syn protein aggregation, phosphorylation, seeding, and uptake.
Diego Ploper received his master’s degree in chemistry at the University of Tucuman. He obtained his Ph.D in Biological Chemistry at the University of California, Los Angeles (2015) focused on Signaling Crosstalk between the Wnt, BMP and Endolysosomal Pathways in Development and Disease under the supervision of Prof. Edward De Robertis. Currently, he is working at Instituto de Investigaciones en Medicina Molecular y Celular Aplicada del Bicentenario, Tucumán.